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Analysis of urine and serum samples Separation of antipyrine and benzocaine in ear drops Global High-performance Liquid Chromatography(HPLC) Market Professional Survey Report 2018 - This report studies High-performance Liquid Chromatography(HPLC) in Global market, especially in North America, China, Europe, Southeast Asia, Japan and India, with production, revenue, consumption, import and export in these regions, from 2013 to 2018, and forecast to 2025. | PowerPoint PPT presentation | free to view 1 HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) Chapter 28 NOTE: HPLC came about because not all compounds can be vaporized and analyzed on a GC Source: D.C. Harris, Exploring Chemical Analysis, 2nd ed. (2001) Major Separation Modes of HPLC A Review There are four major separation modes that are used to separate most compounds: ¾Reversed-phase chromatography (most popular) ¾Normal-phase and adsorption chromatography ¾Ion exchange chromatography ¾Size exclusion chromatography ….Let’s look briefly at each mode Solvent and reagent - HPLC grade Mixed mobile phase or buffer Volume contraction effects – mixing water-miscible solvents pH adjustment, addition of non -ionic additives Filter immediately before use Pore size 0.45um to 0.8um Types of filter: Nylon – hydrophilic (high content of water/aqueous) PTFE – hydrophobic, chemically resistant HPLC seminar 1.

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High Priced Liquid Chromatography. It is column chromatography. It is Liquid Chromatography.

It is important to understand the individual component parts and how each one contributes to the overall reliability of the analysis. High Performance Liquid Chromatography (HPLC) is one of the most widely used techniques for identification, quantification and purification of mixtures of organic compounds.
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Stationary phase: Can be a solid (LSC) or a liquid (LLC) A mixture of compounds injected at one end of the column separates as the compounds pass through.

Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography.
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Internal diameter of column - the smaller in diameter, the higher in sensitivity 2. Pump pressure - the higher in pressure, the higher in separation 3.